Bioreactor
Analytical technique description:
The bioreactor (or fermenter) is a controlled device that allows the growth of microorganisms under optimal conditions. This bioreactor may continuously measure the following parameters: temperature, pH, oxygen, conductivity and NH4+. The temperature of the nutrient medium is maintained by a coil. Nutrients may be continuously added to the fermenter (a fed-batch system), or may be charged into the reactor at the beginning of fermentation. The pH of the medium is measured and could be adjusted with small amounts of acid or base, depending upon the fermentation. For aerobic (and some anaerobic) fermentations, air (or purified oxygen) could be added continuously.
Application:
Production of microbial biomass (bacteria, cyanobacteria, green algae, diatoms) necessary, e.g. for the bioremediation treatments in the cultural heritage field, or as an inoculum for testing the susceptibility of conservation products and biocides to biodeterioration.
Basics:
The environmental conditions that influence the growth and productivity of the organisms inside the bioreactor are continuously monitored and, a software permits to maintain some specific condition (e.g. constant pH during the experiment), in order to have the optimal growing conditions for the chosen microorganism.
Instrumental details:
M2M, Oxysens 120, Ammonium Electrode ISE Oakton, pH Hamilton, temperature probe, conductivity probe, USB-Management Software Tool M2M (Florence Unit).
ICVBC - AREA DI RICERCA CNR DI FIRENZE
Via Madonna del Piano, 10 - 50019 Sesto Fiorentino (FI)
Tel. 055 5225484 - Fax 055 5225483
Institute for the Conservation and Valorization of Cultural Heritage