BIOLUMINOMETER
Analytical technique description:
The Bioluminometer allows to instantly verify the microbial contamination of a surface or a liquid, with a bioluminescent reaction.
II Bioluminometer "read" a light signal that is an output from a sampling device that was passed on the contaminated surface or in the water to be analyzed. During the sampling, the sampling device picks up a cellular molecule, I'ATP (adenosine triphosphate) which is present in all living organisms. When I'ATP is placed in contact with the reaction mixture present in the sampling tube (containing luciferin and luciferase), a reaction takes place that leads to the production of light (photons). This light is measured by the luminometer and converted into a numerical value shown on the display. Higher the microbial contamination is, higher the amount of ATP collection was and, consequently, higher the light produced and the value measured by luminometer is.
Application:
In-situ application for detection of microbial activity on all types of cultural heritage materials.
In biocide dosing programs for controlling the microbiodeteriogens (checking the biocide/disinfectant efficiency in time, establish the minimal inhibitory concentration).
Basics: ATP (adenosine triphosphate) is a molecule found in all around living cells, and it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally occurring firefly enzyme luciferase using a luminometer (called also bioluminometer). The amount of light produced is directly proportional to the amount of living organisms present in the sample.
 
Instrumental details:
3M™ Clean-Trace™ NG Luminometer, Swabs 3M Clean-Trace Surface3M™,  Clean-Trace™ Water ATP (Florence Unit).
ICVBC - AREA DI RICERCA CNR DI FIRENZE
Via Madonna del Piano, 10 - 50019 Sesto Fiorentino (FI)
Tel. 055 5225484 - Fax 055 5225483
Institute for the Conservation and Valorization of Cultural Heritage